Other Names: Cherry fluorescent protein; dsRed, red fluorescent protein, tdTomato antibody.
Other Names: Cherry fluorescent protein; dsRed, red fluorescent protein, tdTomato antibody.
Concentration: 3 mg/ml
Antibody Type: Primary
Target Type: anti-RFP
Accession Number: N/A
Host Species: Goat
Immunogen Species: Purified recombinant RFP peptide produced in E. coli.
Immunogen Type: Recombinant protein
Immunogen: Purified recombinant RFP peptide produced in E. coli.
Isotype: IgG
Clonality: Polyclonal
Confirmed Reactive Species: Red Fluorescent Protein (dsRed), mCherry, mOrange, mPlum, mRFP, mStrawberry and tdTomato
Specificity Detail: In 293HEK cells transfected with cds plasmid detects a band of 29 kDa by Western blot. This antibody also recognizes very well tdTomato and does not cross-react to GFP (green fluorescent protein).
Buffer: PBS, 20% glycerol and 0.05% sodium azide
Purification: Epitope affinity purified
Conjugation: Unconjugated
Format: PBS, 20% glycerol and 0.05% sodium azide
Application: WB, IF, IHC-P, IHC-F, IEM
Concentration/Dilution: WB:1:500-1:2,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500
Storage: Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
References:
1. D Jiang, D Correa-Gallegos, S Christ, et al. Nature Cell Biology 2018 Apr. PMID:29593327,
2. Tian DS, Peng J, Murugan M, et al. J Neurosci. 2017 Aug. PMID:28716963,
3. Semerci F, Choi WT, Bajic A, et al. Elife. 2017 Jul 12. PMID:28699891