EvaGreen® Plus Dye is a green fluorescent nucleic acid dye that is essentially nonfluorescent by itself, but becomes highly fluorescent upon binding to dsDNA. EvaGreen® Plus Dye improves upon these properties with lower background fluorescence and increased brightness upon binding dsDNA. The dye has an excitation maximum that is a better fit for common qPCR instruments, resulting in improved sensitivity. As a result, EvaGreen® Plus Dye can give earlier Ct values and better signal discrimination. These properties offer potential advantages for digital PCR and isothermal amplification applications.