Human Holo TC (Holotranscobalamin) Active vitamin B12 ELISA Kit - 96-wells plate

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516.00 €

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    Assay range: 20pmol/L-800pmol

    Sensitivity: 5pmol/

    Standard: 900pmol/L


    Principle:


    This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Holo TC. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for Holo TC is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Holo TC and HRP conjugated Holo TC antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Holo TC. You can calculate the concentration of Holo TC in the samples by comparing the OD of the samples to the standard curve.


    User manual: