pCDNA3.1 EYFP H148Q-I152L

Catalog No. PVT10772
Packing 2ug

pCDNA3.1 EYFP H148Q-I152L Information

Promoter: CMV

Replicator: pUC

Growth Strain: DH5a

Culture temperature: 37℃

Host: Mammalian cells

Plasmid uses: Protein expression

Segment type: ORF

Fragment species:

Prokaryotic resistance: Amp

Selectable markers: Neo/G418

Fluorescent marking: yellow

pCDNA3.1 EYFP H148Q-I152L Desccription
pCDNA3.1 EYFP H148Q-I152L is a  Mammalian fluorescent plasmid.

pCDNA3.1 EYFP H148Q-I152L Sequence

LOCUS Exported 6178 bp ds-DNA circular SYN 27-SEP-2017

DEFINITION synthetic circular DNA

ACCESSION .

VERSION .

KEYWORDS .

SOURCE synthetic DNA construct

ORGANISM synthetic DNA construct

REFERENCE 1 (bases 1 to 6178)

AUTHORS .

TITLE Direct Submission

JOURNAL Exported Sep 27, 2017

FEATURES Location/Qualifiers

source 1..6178

/organism="synthetic DNA construct"

/mol_type="other DNA"

enhancer 202..581

/label=CMV enhancer

/note="human cytomegalovirus immediate early enhancer"

promoter 582..785

/label=CMV promoter

/note="human cytomegalovirus (CMV) immediate early

promoter"

promoter 830..848

/label=T7 promoter

/note="promoter for bacteriophage T7 RNA polymerase"

regulatory 927..936

/regulatory_class="other"

/note="vertebrate consensus sequence for strong initiation

of translation (Kozak, 1987)"

CDS 933..1652

/codon_start=1

/product="enhanced YFP"

/label=EYFP

/note="mammalian codon-optimized"

/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL

KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD

GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSQNVYLMADKQKNGIK

VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL

EFVTAAGITLGMDELYK"

polyA_signal 1745..1969

/label=bGH poly(A) signal

/note="bovine growth hormone polyadenylation signal"

rep_origin 2015..2443

/direction=RIGHT

/label=f1 ori

/note="f1 bacteriophage origin of replication; arrow

indicates direction of (+) strand synthesis"

promoter 2457..2786

/label=SV40 promoter

/note="SV40 enhancer and early promoter"

rep_origin 2637..2772

/label=SV40 ori

/note="SV40 origin of replication"

CDS 2853..3647

/codon_start=1

/gene="aph(3')-II (or nptII)"

/product="aminoglycoside phosphotransferase from Tn5"

/label=Neo

/note="confers resistance to neomycin, kanamycin, and G418

(Geneticin(R))"

/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

polyA_signal 3821..3942

/label=SV40 poly(A) signal

/note="SV40 polyadenylation signal"

rep_origin complement(4393..4978)

/direction=LEFT

/label=ori

/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

replication"

CDS complement(5149..6009)

/codon_start=1

/gene="bla"

/product="beta-lactamase"

/label=AmpR

/note="confers resistance to ampicillin, carbenicillin, and

related antibiotics"

/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

LIKHW"

promoter complement(6010..6114)

/gene="bla"

/label=AmpR promoter

ORIGIN

1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt

61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct

121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg

181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta

241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg

301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc

361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa

421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca

481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta

541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt

601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg

661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca

721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca

781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc

841 actataggga gacccaagct ggctagcgtt taaacttaag cttgcatgcc tgcaggtcga

901 ctctagagga tccccgggta ccggtcgcca ccatggtgag caagggcgag gagctgttca

961 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg

1021 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca

1081 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc

1141 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc

1201 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc

1261 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg

1321 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaga

1381 acgtctatct gatggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc

1441 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg

1501 gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca

1561 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga

1621 tcactctcgg catggacgag ctgtacaagt aaagcggccg cgactctaga attctgcaga

1681 tatccagcac agtggcggcc gctcgagtct agagggcccg tttaaacccg ctgatcagcc

1741 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg

1801 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat

1861 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag

1921 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg

1981 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc

2041 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc

2101 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct

2161 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa

2221 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc

2281 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca

2341 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat

2401 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt

2461 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc

2521 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta

2581 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc

2641 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta

2701 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct

2761 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat

2821 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag

2881 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg

2941 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca

3001 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc

3061 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg

3121 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg

3181 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta

3241 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag

3301 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac

3361 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg

3421 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg

3481 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg

3541 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg

3601 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg

3661 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc

3721 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct

3781 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta

3841 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact

3901 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc

3961 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta

4021 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc

4081 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg

4141 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg

4201 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg

4261 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa

4321 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc

4381 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc

4441 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag

4501 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct

4561 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta

4621 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc

4681 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc

4741 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt

4801 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct

4861 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc

4921 tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga

4981 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg

5041 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg

5101 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt

5161 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact

5221 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat

5281 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg

5341 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg

5401 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat

5461 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc

5521 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt

5581 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc

5641 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga

5701 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc

5761 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa

5821 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta

5881 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg

5941 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg

6001 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat

6061 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt

6121 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggt

//

Caution:
1.  This product is FOR RESEARCH USE ONLY!
2.  The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.