pRK5-HA
Catalog No. PVT11027
Packing 2ug
pRK5-HA Information
Function: Mammal expression plasmid
Resistance Amp
Competent cells: DH5alpha
Culture temperature: 37degrees centigrade
Replicon: pUC
Copy number: high
Promoter: CMV
pRK5-HA Multiple cloning sites
pRK5-HA Sequence
LOCUS Exported 4727 bp ds-DNA circular SYN 20-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..4727
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 14..393
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 394..597
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 828..846
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
regulatory 963..972
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 975..1001
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
misc_feature 1001..1072
/label=MCS
polyA_signal 1111..1245
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter 1381..1599
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 1450..1585
/label=SV40 ori
/note="SV40 origin of replication"
rep_origin 1848..2303
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2585..2689
/gene="bla"
/label=AmpR promoter
CDS 2690..3550
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3721..4310
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
901 acctcggttc tatcgattga attcccgaac cgacagtcgg tctcttcacc aaggccattc
961 gcgccaccat gggctacccc tatgatgtgc ctgactacgc agatctcaat ggtggtggtg
1021 gtgggtcgac gtacccgggc gtcaagcttg ctagatatcc gcatgcggcc gctaagtaag
1081 taaggatccc cagcttggcc gccatggccc aacttgttta ttgcagctta taatggttac
1141 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
1201 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcgggaa ttaattcggc
1261 gcagcaccat ggcctgaaat aacctctgaa agaggaactt ggttaggtac cttctgaggc
1321 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
1381 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
1441 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
1501 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
1561 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctgttaac agcttggcac
1621 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
1681 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
1741 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
1801 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag
1861 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
1921 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
1981 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
2041 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
2101 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
2161 aactggaaca acactcaacc ctatctcggg ctattctttt aatttataag ggattttgcc
2221 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
2281 caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc
2341 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
2401 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
2461 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
2521 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
2581 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
2641 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
2701 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
2761 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
2821 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
2881 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
2941 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
3001 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
3061 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
3121 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
3181 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
3241 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
3301 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
3361 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
3421 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
3481 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
3541 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
3601 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
3661 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
3721 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
3781 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
3841 cagcagagcg cagataacca aatactgttc ttctagtgta gccgtagtta ggccaccact
3901 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
3961 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
4021 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
4081 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
4141 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
4201 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
4261 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
4321 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
4381 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
4441 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
4501 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
4561 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
4621 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
4681 gataacaatt tcacacagga aacagctatg acatgattac gaattaa
//
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.