pGreen II-0800-Luc
Catalog No. PVT11187
Packing 2ug
pGreenII 0800-Luc Information
Promoter: CaMV 35S, T7
Replicon: pSa ori, ori
Plasmid classification: plant series, protein overexpression vector
Plasmid size: 6382bp
Prokaryotic resistance: Kan
Clonal strain: DH5 alpha
Culture conditions: 37 C, aerobic LB
Expression host: plant cells
5'sequencing primers: 35S:GACGCACAATCCCACTATCC
Primers for 3'sequencing: primers designed based on sequences
pGreenII 0800-Luc Description
We describe novel plasmid vectors for transient gene expression usingAgrobacterium, infiltrated into Nicotiana benthamiana leaves. We have generated a series of pGreenIIcloning vectors that are ideally suited to transient gene expression, by removing elements of onventional binary vectors necessary for stable transformation such as transformation selection genes.
pGreenII 0800-Luc Multiple cloning site
pGreenII 0800-Luc Sequence
LOCUS Exported 6382 bp ds-DNA circular SYN 14-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 8
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6382)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Wednesday, September 14, 2016 from SnapGene Viewer 3.2.1
FEATURES Location/Qualifiers
source 1..6382
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 542..564
/note="LB T-DNA repeat"
/note="left border repeat from nopaline C58 T-DNA
(truncated)"
promoter 626..971
/note="CaMV 35S promoter"
/note="strong constitutive promoter from cauliflower mosaic
virus"
CDS 982..1917
/codon_start=1
/product="luciferase from the anthozoan coelenterate
Renilla reniformis (sea pansy)"
/note="Rluc"
/translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
polyA_signal 1974..2150
/note="CaMV poly(A) signal"
/note="cauliflower mosaic virus polyadenylation signal"
primer_bind 2314..2330
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
promoter 2337..2355
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 2381..2397
/note="KS primer"
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2431..2447)
/note="SK primer"
/note="common sequencing primer, one of multiple similar
variants"
CDS 2479..4131
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/note="luciferase"
/note="enhanced luc+ version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
polyA_signal 4188..4364
/note="CaMV poly(A) signal"
/note="cauliflower mosaic virus polyadenylation signal"
misc_feature 4374..4398
/note="RB T-DNA repeat"
/note="right border repeat from nopaline C58 T-DNA"
rep_origin complement(4489..5077)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5248..6063)
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
rep_origin 6354..407
/note="pSa ori"
/note="origin of replication from bacterial plasmid pSa"
ORIGIN
1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgccag cgcctttgcc
421 cacaacccgg cggccggccg caacagatcg ttttataaat tttttttttt gaaaaagaaa
481 aagcccgaaa ggcggcaacc tctcgggctt ctggatttcc gatccccgga attagagatc
541 ttggcaggat atattgtggt gtaacgttat cgtaccccta ctccaaaaat gtcaaagata
601 cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt tcgggaaacc
661 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
721 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatgcctctg
781 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
841 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
901 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
961 tggagaggac agcccaccac catgacttcg aaagtttatg atccagaaca aaggaaacgg
1021 atgataactg gtccgcagtg gtgggccaga tgtaaacaaa tgaatgttct tgattcattt
1081 attaattatt atgattcaga aaaacatgca gaaaatgctg ttattttttt acatggtaac
1141 gcggcctctt cttatttatg gcgacatgtt gtgccacata ttgagccagt agcgcggtgt
1201 attataccag accttattgg tatgggcaaa tcaggcaaat ctggtaatgg ttcttatagg
1261 ttacttgatc attacaaata tcttactgca tggtttgaac ttcttaattt accaaagaag
1321 atcatttttg tcggccatga ttggggtgct tgtttggcat ttcattatag ctatgagcat
1381 caagataaga tcaaagcaat agttcacgct gaaagtgtag tagatgtgat tgaatcatgg
1441 gatgaatggc ctgatattga agaagatatt gcgttgatca aatctgaaga aggagaaaaa
1501 atggttttgg agaataactt cttcgtggaa accatgttgc catcaaaaat catgagaaag
1561 ttagaaccag aagaatttgc agcatatctt gaaccattca aagagaaagg tgaagttcgt
1621 cgtccaacat tatcatggcc tcgtgaaatc ccgttagtaa aaggtggtaa acctgacgtt
1681 gtacaaattg ttaggaatta taatgcttat ctacgtgcaa gtgatgattt accaaaaatg
1741 tttattgaat cggacccagg attcttttcc aatgctattg ttgaaggtgc caagaagttt
1801 cctaatactg aatttgtcaa agtaaaaggt cttcattttt cgcaagaaga tgcacctgat
1861 gaaatgggaa aatatatcaa atcgttcgtt gagcgagttc tcaaaaatga acaataattc
1921 tagccggtac gctgaaatca ccagtctctc tctacaaatc tatctctctc tattttctcc
1981 ataaataatg tgtgagtagt ttcccgataa gggaaattag ggttcttata gggtttcgct
2041 catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc
2101 aataaaattt ctaattccta aaaccaaaat ccagtactaa aatccagatc gataacatta
2161 acgtttacaa tttccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
2221 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
2281 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat
2341 acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcgac ggtatcgata
2401 agcttgatat cgaattcctg cagcccgggg gatccactag ttctagagcg gccgccaccg
2461 cggtggagat cgaattccat ggaagacgcc aaaaacataa agaaaggccc ggcgccattc
2521 tatccgctgg aagatggaac cgctggagag caactgcata aggctatgaa gagatacgcc
2581 ctggttcctg gaacaattgc ttttacagat gcacatatcg aggtggacat cacttacgct
2641 gagtacttcg aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca
2701 aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc
2761 gcgttattta tcggagttgc agttgcgccc gcgaacgaca tttataatga acgtgaattg
2821 ctcaacagta tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa
2881 aaaattttga acgtgcaaaa aaagctccca atcatccaaa aaattattat catggattct
2941 aaaacggatt accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc
3001 ggttttaatg aatacgattt tgtgccagag tccttcgata gggacaagac aattgcactg
3061 atcatgaact cctctggatc tactggtctg cctaaaggtg tcgctctgcc tcatagaact
3121 gcctgcgtga gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat
3181 actgcgattt taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga
3241 tatttgatat gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgtttctg
3301 aggagccttc aggattacaa gattcaaagt gcgctgctgg tgccaaccct attctccttc
3361 ttcgccaaaa gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct
3421 ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca
3481 ggtatcaggc aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag
3541 ggggatgata aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg
3601 gatctggata ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt
3661 cctatgatta tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag
3721 gatggatggc tacattctgg agacatagct tactgggacg aagacgaaca cttcttcatc
3781 gttgaccgcc tgaagtctct gattaagtac aaaggctatc aggtggctcc cgctgaattg
3841 gaatccatct tgctccaaca ccccaacatc ttcgacgcag gtgtcgcagg tcttcccgac
3901 gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg
3961 gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga
4021 ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa
4081 atcagagaga tcctcataaa ggccaagaag ggcggaaaga tcgccgtgta attctagaga
4141 attcgctgaa atcaccagtc tctctctaca aatctatctc tctctatttt ctccataaat
4201 aatgtgtgag tagtttcccg ataagggaaa ttagggttct tatagggttt cgctcatgtg
4261 ttgagcatat aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa
4321 atttctaatt cctaaaacca aaatccagta ctaaaatcca gatccactag ccttgacagg
4381 atatattggc gggtaaacta agtcgctgta tgtgtttgtt tgagatctca tgtgagcaaa
4441 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
4501 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
4561 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
4621 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
4681 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
4741 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
4801 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
4861 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
4921 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaagaag
4981 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
5041 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
5101 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
5161 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
5221 tatatatgtg taacattggt ctagtgatta gaaaaactca tcgagcatca aatgaaactg
5281 caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga
5341 aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat
5401 tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc
5461 aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa gtttatgcat
5521 ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc
5581 aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt
5641 aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc
5701 aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg ttttccctgg
5761 gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg
5821 aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa caacattggc
5881 aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg
5941 gtagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc
6001 agcatccatg ttggaattta atcgcggcct tgagcaagac gtttcccgtt gaatatggct
6061 cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc atgatgatat
6121 atttttatct tgtgcaatgt aacatcagag attttgagac acaacgtggc tttgttgaat
6181 aaatcgaact tttgctgagt tgaaggatca gatcacgcat cttcccgaca acgcagaccg
6241 ttccgtggca aagcaaaagt tcaaaatcac caactggtcc acctacaaca aagctctcat
6301 caaccgtggc tccctcactt tctggctgga tgatggggcg attcaggcga tccccatcca
6361 acagcccgcc gtcgagcggg ct
//
*Caution
Product is for research use only!