![[AFG-E9107] AffiELISA® Human NTXI (Cross Linked N-Telopeptide Of Type I Collagen) ELISA Kit - 96-wells plate](/web/image/product.product/8474/image_1024/%5B0855-ELK1843-96T%5D%20%5BAFG-E9107%5D%20AffiELISA%C2%AE%20Human%20NTXI%20%28Cross%20Linked%20N-Telopeptide%20Of%20Type%20I%20Collagen%29%20ELISA%20Kit%20-%2096-wells%20plate?unique=f3a8988)
| Product name: | Human NTXI(Cross Linked N-Telopeptide Of Type I Collagen) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | NTX-I; NTX1 |
| Assay Type: | Competitive Inhibition |
| Sensitivity: | 0.94 ng/mL |
| Standard: | 200 ng/mL |
| Detection Range: | 3.13-200 ng/mL |
| Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length: | 2h |
| Research Area: | Metabolic pathway; Bone metabolism; |
| Test principle: | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human NTXI protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NTXI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NTXI in the samples is then determined by comparing the OD of the samples to the standard curve. |