Vitamin A (Vit.A) ELISA Kit - 96 wells plate

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    Reactivity:Panspecies
    Alternative Names:VA, Vit. A
    Assay Type:Competitive Inhibition
    Sensitivity:9.38 ng/mL
    Standard:1000 ng/mL
    Detection Range:15.63-1000 ng/mL
    Sample Type:serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
    Assay Length:2h
    Research Area:Metabolic pathway; Endocrinology; Hormone metabolism;
    Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with VA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to VA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VA in the samples is then determined by comparing the OD of the samples to the standard curve.