K599 Chemically Competent Cell - 10 tubes (100μL/tube)
The genotype of K599 Chemically Competent Cell is Agrobacterium rhizogenes (str R) pRi2659 (agropine type). Agrobacterium rhizogenes is a Gram-negative soil bacterium that can infect most dicotyledons, a few monocotyledons and some gymnosperms. The K599 Agrobacterium rhizogenes strain contains pRi2659 agrobacterium-type Ri plasmid with a broad host range (cucurbitaceae, leguminosae, solanaceae, etc.) and streptomycin resistance.
Applications: K599 Chemically Competent Cell is suitable for transgenic operations of cucurbitaceae, leguminosae, solanaceae and other plants.
1. Volume of DNA from ligation mix should not exceed 1/10 of the cell mixture; DNA for transformation should be purified and free of organic substances such as ethanol.
2. Do not pipette or vortex cells.
3. Plating volume can be adjusted accordingly.
4. Please avoid excessive use of rifampicin. Maximum concentration of rifampicin in selection is 25 μg/mL.
Transformation efficiency of K599 Chemically Competent Cell using pCAMBIA2301 plasmid with 50 μg/mL kan is >10^4 cfu/μg DNA. Transformation efficiency is reduced to half when the plate contains 50 μg/mL kan and 20 μg/mL rif.
Storage: Store at - 80 °C for 12 months.